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KMID : 0545120150250050629
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 5 p.629 ~ p.636
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Bioactive Metabolites Produced by Pseudonocardia endophytica VUK-10 from Mangrove Sediments: Isolation, Chemical Structure Determination and Bioactivity
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Usha Kiranmayi Mangamuri
Muvva Vijayalakshmi
Sudhakar Poda
Bramanandam Manavathi
Ch. Bhujangarao
Y. Venkateswarlu
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Abstract
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Chemical investigation of the actinobacterial isolate Pseudonocardia endophytica VUK-10 has led to the segregation of two known bioactive compounds, namely 4-(2-acetamidoethyl) phenyl acetate and 4-((1, 4-dioxooctahydropyrrolo [1, 2-a] pyrazin-3-yl) methyl) phenyl acetate. The strain was isolated from a sediment sample of the Nizampatnam mangrove ecosystem, south coastal Andhra Pradesh, India. The chemical structure of the active compounds was established on the basis of spectroscopic analysis, including 1H NMR and 13C NMR spectroscopies, FTIR, and EIMS. The antimicrobial and cytotoxic activities of the bioactive compounds produced by the strain were tested against opportunistic and pathogenic bacteria and fungi and on MDA-MB-231, OAW, HeLa, and MCF-7 cell lines. The compounds exhibited antimicrobial activities against gram-positive and gram-negative bacteria and fungi and also showed potent cytotoxic activity against MDA-MB-231, OAW, HeLa, and MCF-7 cell lines. This is the first example for this class of bioactive compounds isolated from Pseudonocardia of mangrove origin.
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KEYWORD
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Mangrove Ecosystem, Pseudonocardia endophytica, Bioactive Compounds, 4-(2-acetamidoethyl) phenyl acetate, 4-((1, 4-dioxooctahydropyrrolo [1, 2-a] pyrazin-3--yl) methyl) phenyl acetate
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